PCR Protocols: Current Methods and Applications
Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White (auth.), Bruce A. White (eds.)
The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols. Key Topics and Techniques: basic startup procedures for PCR • PCR in detecting DNA and RNA • PCR in DNA synthesis and mutagenesis • PCR cloning of DNA • selection of primers • radioactive and nonradioactive labeling • quantitation of PCR products by HPLC • chromosome assignment • PCR mapping of HLA genes • sequencing of PCR products • genomic footprinting • quantitation of tumor gene expression • screening of phage libraries • SSP-PCR and genome walking • subcloning of PCR products.
Categories:
Year:
1993
Publisher:
Humana Press
Language:
english
Pages:
387
ISBN 10:
0896032442
Series:
Methods in Molecular Biology 15
File:
PDF, 21.97 MB
IPFS:
,
english, 1993